Real Time PCR Master Mixes

Real-time PCR master mixes are batch mixtures of PCR reagents at optimal concentrations that can be prepared and divided among multiple PCR tubes/plates. They reduce pipetting and risk of contamination, save time, and limit possible mixing errors.

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376

Thermo Scientific™ DyNAmo ColorFlash SYBR Green qPCR Kit

Offers equal performance to the DyNAmo Flash SYBR Green qPCR Kit. In addition, it incorporates an innovative multi-color system that ensures correct pipetting.

Pricing & Availability
Specifications

Polymerase	Tbr DNA polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
Sample Type	DNA, dsDNA
Reaction Speed	Fast
Kit Type	DyNAmo ColorFlash SYBR Green qPCR Kit
Concentration	2X (qPCR Mix), 50X (Dye), 40X (Buffer)
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	DyNAmo Flash
Detection Method	SYBR

377

Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix, low ROX

Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Pricing & Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Product Type	qPCR Master Mix
For Use With (Equipment)	7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	Luminaris
Detection Method	SYBR

378

Thermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Pricing & Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	Maxima
Detection Method	SYBR

379

Thermo Scientific™ ABsolute Blue QPCR Mix, low ROX

ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments.

Pricing & Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Format	Tube
Product Type	qPCR Mix
For Use With (Equipment)	7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	ABsolute Blue
Detection Method	Primer-probe